Transformation of human umbilical cord blood T cells by human T-cell leukemia/lymphoma virus.

Transmission of human T-cell leukemia virus (HTLV) into human cord blood T cells.

The isolation of HTLV, a type C retrovirus, was first reported from our laboratory from adult patients with T-cell malignancies [4, 10, 111. HTLV has now been isolated from a number of T-cell leukemia/lymphoma patients from various parts of the world, including the United States, Caribbean, Israel, and Japan [14]. An identical or extremely closely related type C retrovirus was subsequently isol...

Hypoxia Preconditioning Promotes Survival And Clonogenic Capacity Of Human Umbilical Cord Blood Mesenchymal Stem Cells

Background: In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate due to cellular senescence, so providing a strategy for increasing the stem cell survival is necessary.  Methods: In this investigation, MSCs characterized by flow cytome...

Ex vivo expansion of human umbilical cord blood-derived T-lymphocytes with homologous cord blood plasma.

This study was designed to establish a more effective and safe culture system for adoptive immunotherapy by investigating the use of homologous cord blood plasma (HCBP) instead of fetal bovine serum (FBS), which has various limitations including ethical problems for the ex vivo expansion of human umbilical T lymphocytes. Fresh human umbilical mononuclear cell fractions were isolated by Ficoll-H...

Study of the Association Between Human T-cell Lymphotropic Virus Type I Infection and Lichen Planus

Molecular characterization of early human T/NK and B-lymphoid progenitor cells in umbilical cord blood.

The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34(+)CD45RA(hi)CD7(+) hematopoietic progenitor cell (HPC) population with that of CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures, and cul...